Lect 7 Microbial genetics 1. Genetic Material DNA & RNA DNA=deoxyribonucleic acid RNA=ribonucleic acid Basic building blocks: Nucleotides Phosphate group Pentose sugar Nitrogenous base 2. Structure of DNA Double stranded (double helix) Chains of nucleotides 5’ to 3’ (strands are anti-parallel) Complimentary base pairing ◦ A-T ◦ G-C #Prokaryotic chromosome 3. DNA Replication Semiconservative ◦ Replication fork Single origin Bidirectional 2 Leading strands 2 Lagging strands Enzymes Helicases DNA polymerases 5’ to 3’ I for leading strand II RNA primer III for lagging strand DNA ligase DNA gyrase DNA is unzipped by unwinding enzyme helicase RNA primer join at the replication site This primer gives signal to enzyme DNA polymerase to synthesize new DNA strand • One new DNA strand (Leading strand) is synthesized continuously in the 5’ 3’ direction • In contrast, the lagging strand is synthesized...