学霸笔记

CLINICAL MICROBIOLOGY 1 001 Introduction to Bacterial Identification Contents Bacterial Taxonomy Steps in Diagnostic Isolation and Identification of Bacteria Isolation and Identification Method ofs Isolation and Culturing of Microorganism Microscopic Examination Biochemical Testing of Microorganism Antimicrobial Sensitivity Testing (AST) 1. Introduction Infectious diseases caused by different bacteria have a variety of clinical courses and consequences Isolation and identification of bacteria aids treatment Susceptibility testing of isolates (e.g: establishing Minimum Inhibitory Concentration, MIC) can help in selection of antibiotics for therapy Recognizing that certain species (or strains) are being isolated atypically may suggest that a disease outbreak has occurred 2. Bacterial Taxonomy Taxonomy is the practice and science of classification Family is a group of related genera Genus is a group of related species Species is a group of related strains Type means sets of strain within ...

Question bank https://www.docdroid.net/jKqtsHZ/clinical-chemistry-mcqs-doc Final preparation (refer to past year questions & assignments) Section B  1. Blood analysis  How is serum prepared (5)  Serum is the fluid portion that remains after blood has been allowed to clot   Serum is obtained from clotted blood that has not been mixed with anticoagulant The sample is then centrifuged, yielding serum Serum contains albumin and globulin  Serum can be collected in serum separator tubes that contain a substance that form a barrier between serum and cells during centrifuge  Steps to avoid hemolysis (5) Do not collect a specimen in a hematoma  Do not centrifuge specimen for a prolonged period of time  Avoid contamination ( allow collection site to dry after cleaning ) Allow specimen clot completely before centrifuge Replace vacuum tube if air leakage around needle Use 21 and 22 gauge needle for routine collections  Compare serum and plasma (4)...